Flavonoid profile and antioxidant activities of methanolic extract of Hyparrhenia hirta (L.) Stapf.

نویسندگان

  • Hanen Bouaziz-Ketata
  • Nabil Zouari
  • Hichem Ben Salah
  • Moez Rafrafi
  • Najiba Zeghal
چکیده

In this study, we report isolation of flavonoids, viz., 3-O-methylquercetin, tangeritin, luteolin-7-O-glucoside, luteolin, apigenin-7-O-glucoside, apigenin-8-C-glucoside, luteolin-8-C-glucoside, luteolin-6-C-glucoside, diosmetin and catechin from the methanolic extract of Hyparrhenia hirta employing high performance liquid chromatography and liquid chromatography-electrospray ionization-tandem mass spectrometry. The total phenolic content of H. hirta extract was 105.58 ± 0.1 mg gallic acid equivalents/g of plant extract while the total flavonoid content was 45.20 ± 0.2 mg quercetin equivalents/g of plant extract and the total condensed tannin were 72.35 ± 0.7 mg catechin equivalents/g of plant extract by reference to standard curve. The antioxidant activity was assayed through the antioxidant capacity by phosphomolybdenum assay, the reducing power assay and the radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl method. The extract showed dose dependant activity in all the three assays.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The Volatile Compounds and Bioactivity of Achillea sieheana Stapf. (Asteraceae)

The in vitro antioxidant and antimicrobial activities of the essential oil and methanolic extract of Achillea sieheana Staf. (Asteraceae) were investigated in this study. The chemical composition of the essential oil isolated by hydro-distillation from the aerial parts of A. sieheana was analyzed by GC–MS. Camphor (43.36%), Artemisia ketone (25.95%), 1.8-cineole (6.29%) and camphene (4.77%) wer...

متن کامل

The Volatile Compounds and Bioactivity of Achillea sieheana Stapf. (Asteraceae)

The in vitro antioxidant and antimicrobial activities of the essential oil and methanolic extract of Achillea sieheana Staf. (Asteraceae) were investigated in this study. The chemical composition of the essential oil isolated by hydro-distillation from the aerial parts of A. sieheana was analyzed by GC–MS. Camphor (43.36%), Artemisia ketone (25.95%), 1.8-cineole (6.29%) and camphene (4.77%) wer...

متن کامل

Phytochemical and Antioxidant Activities of Methanolic Extract of the Plant Euphorbia thymifolia Linn

The present study was designed for phytochemical and antioxidant activity of methanolic extract of the whole plant Euphorbia thymifolia linn. Belonging to the family Euphorbiaceae. It cures many diseases. The phytochemicals present in this methanolic extract were studied by standard Protocols and the invitro antioxidant activities were studied using different methods i.e., H2O2 scavenging activ...

متن کامل

Antioxidant and antiradical activities of Rosa canina and Rosa pimpinellifolia fruits from West Azerbaijan. Somaye Fattahi, Rashid Jamei* and Siavash Hosseini Sarghein

Rosa canina L. is a member of Rosaceae family, which is well-known for its high phenolic contents. These compounds are known to possess antioxidant and antiradical effects. In order to compare the antioxidant and antiradical activity of wild Rosa phenolic extracts, two wild Rosa species (Rosa canina L. and Rosa pimpinellifolia L.) were selected from Takab, Oshnavieh and Qasemloo Valley of Urmia...

متن کامل

بررسی برخی ویژگی‌های اکولوژیکی گونه مرتعی Hyparrhenia hirta (L.) Stapf به‌منظور احیاء مراتع نیمه‌استپی گرم

بهره‌برداری نادرست و بیش از حد، توان تولید و باردهی گیاهان عمده مرتعی را کاهش داده موجب از بین رفتن بسیاری از آنها و انهدام مراتع شده است. به‌منظور دست‌یابی به اطلاعات و آمار موردی گونه Hyparrhenia hirta (L.) Stapf برای تجدید حیات و توسعه آن و نهایتاَ احیاء مراتع مخروبه، بررسی و انجام مطالعات برخی از ویژگی‌های اکولوژیکی گونه مورد نظر ضروری است. در این تحقیق آت اکولوژی گونه مرتعی Hyparrhenia hirt...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Indian journal of experimental biology

دوره 53 4  شماره 

صفحات  -

تاریخ انتشار 2015